New restriction endonuclease CviRI cleaves DNA at TG/CA sequences.
نویسندگان
چکیده
A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 22 19 شماره
صفحات -
تاریخ انتشار 1994